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Thus, to assess the influence of the 5′-flank, the position of the consensus sequence in the k-mer was fixed and all possible combinations (A, C, G or T) of 5′ flanks were considered. The flanking sequences of different G-boxes significantly affected G-box activity and promoter activity as shown through the use of both native and synthetic promoters to drive expression of gfp. Do not include MeSH terms found below this term in the MeSH hierarchy. Use your Restriction Digest and Ligation skills to insert into a plasmid of choice. mainstays radiator heater review The simpler RAD method was used to generate short DNA tags at each cognate cleavage site. Whilst Flanker overcomes these challenges, users may need to. Jan 16, 2013 · Exogenous fragment sequence and flanking sequence between exogenous fragment and recombinant chromosome of transgenic wheat B72-8-11b were successfully acquired through PCR amplification with cross-matched primers from exogenous genes. To this end, we cloned a 19 kb DNA fragment containing the Chinese hamster elongation factor-1alpha (EF-1alpha) gene, as well as 12 kb of 5' flanking sequence and 4 kb of 3' flanking sequence. To obtain the flanking sequence of the target gene of the transgenic maize event, after the failure of the genome walking method and the whole genome sequencing method, the single-molecule real-time sequencing was used to identify the accurate flanking sequences of the inserted gene. walmart automotive prices Thus, to assess the influence of the 5′-flank, the position of the consensus sequence in the k-mer was fixed and all possible combinations (A, C, G or T) of 5′ flanks were considered. Analysing the flanking sequences surrounding genes of interest is often highly relevant to understanding the role of mobile genetic elements (MGEs) in horizontal gene transfer, particular for antimicrobial resistance genes. How to use flank in a sentence. With her Chief Inspector Armand Gamache series, Penny has created a world t. scooterbug Dec 6, 2008 · The flanking sequence output is shown in fasta format with on-line representation and file and/or text. ….

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